Stefan Paulusch

12 Questions 16 Answers 0 Followers

Questions related from Stefan Paulusch

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

22 February 2021 6,808 3 View

How to measure endogenous miRNA expression from 2 different body compartments ?

Hello RG community, My task is to measure the expression of several microRNAs from total RNA isolated from human body sera. I have a number of TIPS patient (suffering from liver cirrhosis) and...

31 August 2018 9,473 1 View

What is best reference gene when quatifying circulating miRNA from TIPS patient serum ?

Hi there, I received extracted whole RNA from TIPS patient serum and negative healthy controls. All samples contained spiked-in SV40 miRNA already as a reference gene. I performed miRNA reverse...

09 April 2018 5,577 2 View

Is glucuronide CDCA-24G soluble in water or in any other solvent?

Hi guys, I have a question about glucuronide CDCA-24G, maybe someone has already worked with it. I want to perform an experiment in hepatocytes which I have extracted from mouse livers. I do not...

28 February 2018 5,148 2 View

Is the number of clone picking to find integrated restriction site in plasmid equal to general process of insert screening ?

Hi guys, a situation has just come up. I want to integrate a restriction site into a Plasmid (~6.5 kb) by PCR mutagenesis. After PCR (25 cycles), I observed the DNA band on the gel, however very...

28 September 2017 2,686 3 View

Best purchasable Hek293 cell culture medium?

Hi RG community, we started cultivation of Hek293 cells in DMEM GlutaMAX from Gibco plus 10 % FCS and Pen Strep. The cells grow pretty slowly presumably due to the medium composition. We´re...

06 July 2017 2,389 8 View

DpnI digestion of the plasmid for site-directed mutagenesis?

Hi, I´m working on site-directed mutagenesis trying to mutate several short stretches of ~6-7 nt within a 5 kbp plasmid. I designed mutagenesis primers. In 2 PCR reactions the 2 DNA fragments are...

02 May 2017 2,607 3 View

How do miRNA regulate transcription of a gene, especially concerning upregulation?

Hello dear community, I am about to start Investigating transcription Regulation with miRNA and I am particularly interested in the question how miRNA come to upregulate gene Expression. Does...

29 June 2016 3,578 4 View

What if HEPG2 cells were washed mistakenly by 10 X PBS ??

I got my HEPG2 cells passaged to a new cell culture flask and have mistakenly used 10 X PBS for washing instead of 1 X PBS. Although washing took relatively short time how bad might be the...

21 June 2016 9,523 3 View

What might be the problem restreaking a single colony over and over again?

A colleague of mine has some diffuculty with his RT-PCR experiment. He started analyzing the single failure-prone steps telling that he has restreaked the strain of interest due to some growth...

26 October 2015 3,136 7 View

How can I make 2 M or 4 M acetic acid from 100% pure glacial acetic acid?

Hi all, For my plasmid isolation the protocol says to use 2 M acetic acid but our store has only got 100 % pure glacial acetic acid. So how can I make a 2 M acetic acid? Happy for any help!

02 July 2015 3,674 12 View

Hi all, can someone provide me the restriction map of plasmid pMHH1?

Hello everyone, since my supervisor left already I am desperately looking for the restriction map of plasmid pMHH1. I want to start curing my E. coli of its plasmid by using suicide vector pMHH1....

01 July 2015 1,562 7 View