How can I define RNA stability/saturation versus induced protein expression for any given gene?

assuming a case where we see induction in protein expression levels of any gene X due to nutrient stress but the transcript levels getting saturated and plateaued after a minor induction with...

08 September 2015 952 4 View

Has anybody worked on the HT22 cell line?

would like to know how to differentiate them and how are the phenotypic changes? do they give rise to dopaminergic or glutamanergic phenotypes? what would be the agents to induce differentiation...

08 September 2015 1,234 10 View

Need this research article to the earliest.

http://www.nature.com/nature/journal/v381/n6584/abs/381667a0.html

08 September 2014 7,930 1 View

Can I use indirect ELISA using bacterial cells as antigens to see expression of certain surface proteins?

I want to coat bacterial cells at a cell number of 10 (^8). I need help in regards to how to design the strategy for coating of my cells, assuming that each well of the 96 well plate will have...

06 July 2014 9,974 15 View

What is the best way to make cryo stocks for CaCo 2 cells?

06 July 2014 7,297 12 View

Is it possible to design primers which contain wrong bases and amplify the target gene, so that it would not affect in its translation?

For introducing mutations (may be site specific) by just designing primers with changes in few base pairs (may be a triplet).

05 June 2014 1,903 3 View

Can anyone help me with 2D gel electrophoresis?

I am a newbie in 2D gel electrophoresis, my aim is to see the protein expression profile of a bacterium when grown in cold temperature and 37 degrees?

05 June 2014 5,516 8 View

Can Chloroform Lyse gram positive bacteria, such as Bacillus? And will it affect bacteriophages if co-cultured with bacillus?

In a situation where there is a host specific phage,eg. phage specific to bacillus cereus, then chloroform is used in a step for isolating these phages. Could there be any possibility that the...

03 April 2014 9,231 5 View

Is anyone familiar with random mutagenesis?

I am going to do for the first time and I have some basic queries. I need the help of someone who has done it.

02 March 2014 4,294 9 View

How to purify a protein and use it for pulldown when no antibody is available commercially?

I want to purify inV gene from enterobacter, but the issue is there is no primary antibody available at the moment. Generating antibody would require the protein to be purified which is also not...

31 December 2013 2,135 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View