Sabyasachi Dash

11 Questions 55 Answers 0 Followers

Questions related from Sabyasachi Dash

Has anybody worked on the HT22 cell line?

would like to know how to differentiate them and how are the phenotypic changes? do they give rise to dopaminergic or glutamanergic phenotypes? what would be the agents to induce differentiation...

09 September 2015 1,310 10 View

How can I define RNA stability/saturation versus induced protein expression for any given gene?

assuming a case where we see induction in protein expression levels of any gene X due to nutrient stress but the transcript levels getting saturated and plateaued after a minor induction with...

09 September 2015 1,020 4 View

Need this research article to the earliest.

http://www.nature.com/nature/journal/v381/n6584/abs/381667a0.html

09 September 2014 7,999 1 View

Can I use indirect ELISA using bacterial cells as antigens to see expression of certain surface proteins?

I want to coat bacterial cells at a cell number of 10 (^8). I need help in regards to how to design the strategy for coating of my cells, assuming that each well of the 96 well plate will have...

07 July 2014 10,079 15 View

What is the best way to make cryo stocks for CaCo 2 cells?

I want to make stocks of CaCo 2 cells.

07 July 2014 7,369 12 View

Is it possible to design primers which contain wrong bases and amplify the target gene, so that it would not affect in its translation?

For introducing mutations (may be site specific) by just designing primers with changes in few base pairs (may be a triplet).

06 June 2014 2,005 3 View

Can anyone help me with 2D gel electrophoresis?

I am a newbie in 2D gel electrophoresis, my aim is to see the protein expression profile of a bacterium when grown in cold temperature and 37 degrees?

06 June 2014 5,594 8 View

Can Chloroform Lyse gram positive bacteria, such as Bacillus? And will it affect bacteriophages if co-cultured with bacillus?

In a situation where there is a host specific phage,eg. phage specific to bacillus cereus, then chloroform is used in a step for isolating these phages. Could there be any possibility that the...

04 April 2014 9,342 5 View

Is anyone familiar with random mutagenesis?

I am going to do for the first time and I have some basic queries. I need the help of someone who has done it.

03 March 2014 4,364 9 View

How can I interpret SWISS model analysis results?

I need to analyse results from a swiss model server. Can anybody help me interpret the results?

02 February 2014 8,864 0 View

How to purify a protein and use it for pulldown when no antibody is available commercially?

I want to purify inV gene from enterobacter, but the issue is there is no primary antibody available at the moment. Generating antibody would require the protein to be purified which is also not...

01 January 2014 2,202 13 View