Has anybody worked on the HT22 cell line?

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Saleh Alkarim

Hello

Look at papers in links may help you

Good Luck

http://www.ncbi.nlm.nih.gov/pubmed/25206424

http://onlinelibrary.wiley.com/doi/10.1111/cns.12044/pdf

https://www.salk.edu/pdf/otmd/Articles/TM_1033_HT22_cells.pdf

http://www.nature.com/emm/journal/v42/n12/full/emm201083a.html

Sabyasachi Dash

thank you Alkarim..

Atefe Tari

Hi, did you manage to differntiate the HT22 cells?

Hi Atefe,

I tried different combinations to differentiate these cells. However, seems like they significantly express Glutamate receptors/transporters like VGLUT etc.

Classically, these cells have been studied and characterized for Glutamate induced excitotoxicity.

i tried using Neurobasal media with BDNF and retinoic acid. observations were really interesting where i see longer neurites. but i have not tried characterizing what exactly which lineage they have been differentiated into.

Kumarasamy Murali

Dear all, I would like to know why sometimes HT-22 cells are proliferating very slow... As I know that, the proliferation rate was very fast before, but when I freeze and thaw it their rate was really slow... please help me in this aspect... Thanks....

Armando Chierchia

Dear all, I have a question: what is the best method to differentiate the HT22 cell line? I tried with Neurobasal A + N2 supplement for 24h but I don't have a good result. What do you suggest?

Thank's!!

try just Neurobasal media with BDNF

Lucy He

Hi everyone,

I have been differentiating my HT22 cells in NeuroBasal medium with N2 supplement and allowing it to incubate for 48 hours before harvesting the cells. Although the differentiation is successful, I have observed around 50% cell death as well. Can anyone provide insight on this for me? Also I am currently using NeuroBasal medium without phenol red catalog # 12348017 on Thermo Fisher. Is this the best medium to use?

Thank you!

I had used Medium with Phenol red and the supplement was B27. I had also seen some cell death in the range of 10-15%.

Hiteshika Gosain

Hello,

what amount of BDNF or NGF and the B27 supplement was used for differentiation of HT-22 cells? Did anyone find out what kind of neurons these cells are differentiated into? Is it possible to transfect these cells using the calcium-phosphate technique? I want to study the localization of the exocyst complex before and after differentiation? Do you recommend to transfect and then do the differentiation or differentiate and then do the transfection?

Thank you.