For introducing mutations (may be site specific) by just designing primers with changes in few base pairs (may be a triplet).
it is possible. when introducing mutations into the primer sequence it is recommended that the mutation will sit in the middle of the primer, and not in the 3' end of it. 1-3 consecutive mutations will usually work fine, but more than 3 mutations will probably lead to some fine-tuning of your reaction.
Please note that mismatched bases in your primer will effect the annealing phase of the reaction, and might reduce the general product yield.
you can google for the "quick change" site-directed mutagenesis by stratagene for more details and figures.
After obtaining a mutant amplicon, you can proceed to your preferred cloning method (ligation, gibson assembly etc.)
good luck!
As Oded said
it is possible if only one base e.g T and C bcz these two bases can anneal with other C and T respectively. Its depend on annealing temperature and salt concentration..
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