How can I define RNA stability/saturation versus induced protein expression for any given gene?

assuming a case where we see induction in protein expression levels of any gene X due to nutrient stress but the transcript levels getting saturated and plateaued after a minor induction with...

08 September 2015 952 4 View

Has anybody worked on the HT22 cell line?

would like to know how to differentiate them and how are the phenotypic changes? do they give rise to dopaminergic or glutamanergic phenotypes? what would be the agents to induce differentiation...

08 September 2015 1,234 10 View

Can I use indirect ELISA using bacterial cells as antigens to see expression of certain surface proteins?

I want to coat bacterial cells at a cell number of 10 (^8). I need help in regards to how to design the strategy for coating of my cells, assuming that each well of the 96 well plate will have...

06 July 2014 9,974 15 View

What is the best way to make cryo stocks for CaCo 2 cells?

06 July 2014 7,297 12 View

Is it possible to design primers which contain wrong bases and amplify the target gene, so that it would not affect in its translation?

For introducing mutations (may be site specific) by just designing primers with changes in few base pairs (may be a triplet).

05 June 2014 1,903 3 View

Can anyone help me with 2D gel electrophoresis?

I am a newbie in 2D gel electrophoresis, my aim is to see the protein expression profile of a bacterium when grown in cold temperature and 37 degrees?

05 June 2014 5,516 8 View

Can Chloroform Lyse gram positive bacteria, such as Bacillus? And will it affect bacteriophages if co-cultured with bacillus?

In a situation where there is a host specific phage,eg. phage specific to bacillus cereus, then chloroform is used in a step for isolating these phages. Could there be any possibility that the...

03 April 2014 9,231 5 View

Is anyone familiar with random mutagenesis?

I am going to do for the first time and I have some basic queries. I need the help of someone who has done it.

02 March 2014 4,294 9 View

How can I interpret SWISS model analysis results?

I need to analyse results from a swiss model server. Can anybody help me interpret the results?

01 February 2014 8,774 0 View

How to purify a protein and use it for pulldown when no antibody is available commercially?

I want to purify inV gene from enterobacter, but the issue is there is no primary antibody available at the moment. Generating antibody would require the protein to be purified which is also not...

31 December 2013 2,135 13 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View