How can I immobilize DNA probes on nitrocellulose membrane?

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Quantile cointegration estimate?

Dear all, does anyone has the estimation code for Xiao (2009) quantile cointegration test.

28 July 2024 6,133 3 View

Getting standard errors and T-statistics for Dynamic panel data with instrumental variable method?

Dear all, I have a user written estimation code based on the paper dynamic panel data estimations with fixed effect by Galvao(2009). I have seen many papers using this method and pvalue, standard...

22 July 2024 4,588 1 View

How many samples size should I select to compare both groups?

I want to study the differences between two groups: the treatment group and the comparison group. The total population consists of 60000 women in the treatment group, distributed across different...

21 July 2024 669 3 View

Is it appropriate to use ARDL(autoregressive distributed lag model) with environmental Kuznets curve?

The underlying idea behind the environmental Kuznets curve is that GDP per capita initially increases environmental degradation but later reduces the environmental degradation as the result of the...

28 June 2024 949 0 View

Why is stiffness of structure reduced in pushover after gravity case?

So I have a structure with a pile foundation and a gravity load acting on the structure. The demand curve is drawn by running an elastic analysis. Pile-Soil is modelled as p-y, t-z, and q-z...

14 June 2024 5,220 0 View

I want to ask if I suppose to add 200mg of antibiotic in 100ml of water so what is the concentration of this stock solution in microgram?

Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...

08 June 2024 2,285 6 View

Are you interested in submitting papers to a new journal called "InfoScience Trends"?

InfoScience Trends offers comprehensive scientific content that delves into various facets of information science research. This includes but is not limited to topics such as information...

26 April 2024 9,521 1 View

What is the main substance of bone marrow?

Red bone marrow contains blood stem cells that can become red blood cells, white blood cells, or platelets. Yellow bone marrow is made mostly of fat and contains stem cells that can become...

10 April 2024 6,011 1 View

Journal of Renal Injury Prevention. How we can activates its profile in RG?

Dear Sir, Hello I am Hamid Nasri, founder of Journal of Renal Injury Prevention; https://www.journalrip.com/ Please let me know, how we can activates its profile in RG. Thank you Hamid...

30 March 2024 5,569 2 View

Hoe to simulate stability of drydocking in maxsurf?

all know the stability of ships in dry-docking has critical period. the maxsurf suite can simulate the stability behavior in many case of loading and damage scenario. now, How to simulate...

06 March 2024 3,845 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,419 1 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View