What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

How do I delete a publication that I added?

I added two times the same thing by mistake and I need to remove one. Thanks.

11 January 2021 6,118 5 View

What is the easiest and cheapest molecular magnet to synthesize at home and how can it be done?

im looking for a diy at home (home lab) molecular magnet in a chemical suspension that is stable at room temperatures and cheap

30 December 2020 626 1 View

What defines the time it takes to disconnect a molecular switch?

I have been thinking about a material that was made 6 months ago, which is a tetramethylsiloxane linked to a thiazolidinedione and finally to a naphthalene, but I can't find the original paper (if...

30 December 2020 5,347 1 View

Are there any molecular dynamics sofware easy to use for homo lumo interraction in molecular dynamics or some sofware for magnetic interraction spins?

I am working with molecular dynamics in a nanotube but I need easy to use software to be able to homo lumo, molecular dynamics and also charge densities in a chemical doping, I am also looking in...

30 December 2020 7,482 1 View

Vhb 4910 and carbon black as a electrode have some diference between carbon with pdms?

There is a dopant for the vhb 4910 actuator tapes that have acetylene black or carbon black which is carbon grease (carbon black + pdms) commercially, I am making a robotic actuator but I don't...

30 December 2020 3,144 1 View

How can I identify the points that are poking out from a point cloud plot?

I have a cloud plot of data relating two important variables for an ecological analysis. I was defining the "abnormal class" as the data points that are >=20 for the Y-axis and >=20 for the...

22 September 2020 7,679 16 View

Why is the pressure slowly increasing when using a Hiplex Ca column?

We recently installed a Hiplex Ca Duo Column from agilent for carbohydrate determination. It was working fine until a week ago, when the pressure of the column started to increase very slowly. As...

03 September 2020 1,733 2 View

How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

How to make RNA extraction process RNase free inexpensively?

We want to extract RNA from virus samples, so we need to make everything RNase free. However, DEPC or RNase cleaning products are out of the question for us at the moment because of their cost. Is...

02 February 2021 2,343 4 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

What can I do if DNA OD260/280 is either 1.6 or higher than 3?

26 January 2021 7,830 4 View

Can I RNase A treatment my extracted plamid?

I have extracted the plasmid with the Qiagen maxi Endofree kit. the result of gel electrophoresis for this sample showed a band of degraded RNAs. Now, I want to remove RNAs from the eluted plasmid...

25 January 2021 2,958 3 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 2,767 6 View