16 Questions 16 Answers 0 Followers
Questions related from Guillermo Domínguez Huerta
I have a cloud plot of data relating two important variables for an ecological analysis. I was defining the "abnormal class" as the data points that are >=20 for the Y-axis and >=20 for the...
23 September 2020 7,800 16 View
Hi all, I have a collection of nucleotide sequences corresponding to one functional domain of a hallmark gene of viruses. So in principle I am working with relatively-homogeneous functional...
29 March 2020 9,016 3 View
Hi all, I am looking for a way to find RNA virus genome termini in metatranscriptomes. Do you know a database of sequences for RNA virus genome termini? Or if they are certain motifs repeated...
10 November 2019 488 2 View
Hi all, I'm working to extract RNA virus sequences from metatranscriptomic datasets. I observed that it is possible to detect some "virus" signal from non-assembled reads (by translation and...
03 June 2019 5,892 1 View
Hi, I'd like to know what difference makes using FULL PROTEIN SEQUENCES versus PROTEIN CONSERVED DOMAIN SEQUENCES in order to make a phylogenetic tree. In my case, the goal is to put in...
24 April 2019 3,179 4 View
Hi all, I was performing TRIzol extraction protocol to get prokaryotic RNA. Samples were treated with rigorous Turbo DNase treatment. Results were good in terms of quantity (50-100 ng/ul)....
06 February 2019 6,961 2 View
Hi all, I'm looking for the expert opinion from a chemist, since I am only a biologist. I have a set of samples made in the following manner: many litters of seawater were...
04 August 2018 4,077 3 View
Hi all, this one is a (I guess) tricky question... RNA virus discovery from metagenome/metatranscriptome dataset (overall from environmental samples) is particularly difficult because of their...
23 July 2018 4,405 1 View
Hi all, I'm trying to get proteins predicted from novel eukaryotic DNA sequences obtained from metagenomics. I guess protein prediction can be problematic from eukaryotic genomes.... WHAT ARE...
29 May 2018 7,783 4 View
Hi all, I wondering about bioinformatic tools to capture "RNA viruses" from environmental sequence datasets. dsDNA phages are pretty abundant and have relatively conserved hallmark genes....
05 March 2018 4,051 5 View
I want to precipitate a cystovirus, which is en enveloped phage. Any of you know if this kind of viruses should be subjected to different conditions when you precipitate with PEG? I mean: PEG and...
27 January 2018 6,192 1 View
Hi all, I looking for literature evidence for this phenomenon. Apparently, RNA (I'm not sure if also DNA) is cleavaged in a non-enzymatic manner by iron. I would need some theory to support...
20 January 2018 3,137 4 View
Hi all, I know this is a typical phenomenon, but after reading I'm not getting a convincing explanation. I prepared 4 L of a buffer containing 10 mM KH2PO4 and 1 mM MgSO4*7H2O. This time I...
12 January 2018 867 6 View
Beyond human pathogenic viruses (enteroviruses, hepatitis A virus, rotavirus, norovirus)..., which other riboviruses are expected to found in freshwaters from an urban river? I'm looking for...
18 December 2017 7,276 2 View
Get rid of the iron from my samples. Iron chelating capacity of EDTA? Briefly, I have samples consisting of resuspended viruses precipitated with FeCl3. After flocculation of viruses, I resuspend...
11 December 2017 6,831 7 View
Hi, Imagine you got a mutant library of a plasmid DNA generated by random mutagenesis and affecting the whole plasmid sequence. This mutant library is going to be used to transform bacteria and...
29 May 2017 4,659 5 View
