How can I extract protein for SDS-PAGE from okra leaf and seed sample?

01 May 2013 1 9K Report

When I am going to extract protein from okra sample, the mucilage interferes in extraction and also during loading on gel and run. Is there any easy method for removal of mucilage during extraction of before extraction?

Saurabh Gayali

Several papers are there which could help you.

http://www.sciencedirect.com/science/article/pii/030441659190188M

http://docsdrive.com/pdfs/academicjournals/ajft/2011/260-278.pdf

http://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740120104/abstract

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1988.tb07854.x/abstract

http://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740120101/abstract

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