How can I explain a reduction of proteic expression more obvious at 24 of incubation with tested sample to 48h?

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How can I adapt cyclic server model in hospital operations?

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Could you please guide me how can I manually separate genomic and plasmid DNA from algae simultaneously?

Next step is doing qPCR. Thanks

23 April 2024 5,442 5 View

Hi, I am going to isolate genomic and plasmid DNA from algea simultaneously and manually, Do you have any suggestion?

I found a kit that they separated plasmid and genomic DNA form mammalian cell, but I want to extract both plasmid and genomic DNA manually. I also want to use the extracted DNA for qPCR.

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Why am I getting low isotopic enrichment in my expressed protein even though I am using labeled media?

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Do you have references dedicated to the influence of batch for the same steel with the same process and the same composition?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

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Nikola M Stojanovic

Well prolonged cultivation probably is needed for obvious results, plus the cells are probably decrease their viability after 48 also.

Rafael Félix

More information on the experiment would be useful...

What is your sample? Fungal proteins, bacterial proteins, intra or extracellular, ...?

How is the growth curve of your organism? Did you characterize it?

Augusta Jamin

are these infected samples? siRNA-treated?

Li Wang

If these protein are death-related, it should make sense.