Why does kaleidoscope Marker miss Bands in the middle??

We had several times the problem after the transfer: you can detect the yellow 10 kDa Band, the blue 20 kDa Band, the pink 25 kDa Band but after only the pink 75 kDa Band. 15, 37 and 50 kDa are...

02 February 2021 4,936 3 View

Where can I find a dataset for blind text steganalysis?

I'm looking for a dataset for blind text steganalysis readily labeled with stego/non-stego class. I'm just testing few ML/DL algorithms in this domain. Any pointer?

12 January 2021 6,965 5 View

What's difference between NH4+ and NH4+-N, NO2-and NO2-N, NO3- and NO3-N?

Hello, Upon writing my paper, I noticed that most journal papers stated the concentration of all nitrogen compounds in -N , example instead of NH4+ its NH4+-N. I knew that the -N form uses the...

14 December 2020 3,693 3 View

What are the authentic assessment options in this COVID-19 period?

I will presume that pre-COVID-19, lecturers/professors lean heavily towards the traditional (perhaps tested and tried) timed mid-term test, quiz and final exams. However, with many of us having...

01 December 2020 4,544 3 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

In the field of Literature, on what basis do we judge that a particular interpretation is false?

Is there such thing as false interpretation in Literature?

21 September 2020 7,327 31 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

What's the efficient method to synthesis substituted hydroxyapatite ?

Hello all I used to synthesis substituted hydroxyapatite using wet chemical process (Co-precipetation), but it leads me always to a structure full of Impurities, beside the uncomplet...

06 September 2020 1,383 6 View

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I have an AlN sample and the EDX data show me the at% of a single atom such as Al, N and O . In order to measure the at% AlN , I have to use the chemical formula 2AlN = 2Al + N 2 . should I use...

20 August 2020 6,269 3 View

What is the impact of solder mask and Surface finish on antenna performance?

Hello everyone, I am working on the design and fabrication of a butler matrix connected to an array antenna. The structure operates at 3GHz. In fact, during the design, I did not include any...

31 May 2020 1,873 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View