Dear Palani,

You are searching for a simple in vitro assay anti-inflammatory properties for plant extracts.

Inhibition of albumin denaturation

It is a very simple. I think it not only helps you but also will solve your problem.

I am attaching a file here to solve your problem.

Read the article carefully. If you have further any query please feel free to ask.

If my given article really helps you please up vote my answer.

I am looking forward to solve your problem soon.

Regards

SALMAN AHMED

Ph.D FELLOW

There are various methods of assessments, both in vitro and in vivo. For example, in vitro assays such as enzyme inhibitory assays (COX, LOX, iNOs), and cytokine inhibitory assays (Interleukins) could be used. In vivo methods of assessments includes the hot-plate methods and a host of others. Your method of choice depends on the extracts to be tested and the possible molecular targets of the compounds in the extracts to be tested on mediators of inflammation. Hope this helps.

What do you want to do exactly? Evaluation in vitro or in vivo? Inflammation is characterize by the high metabolite level of arachidonic acid which were product through two different enzymatic way: (1)-cyclooxigenase and (2)-lipoxigenase. You need to know what you want to do. You can follow in vitro one of the two way.

determination for the IC50 for both COX 1 and COX 2 in vitro. In vivo assay using for example carrageenan-induced paw edema method

Dear thank you for this contact, i prefer to ask dr. sidig ibrahim abdelwahab

[email protected]; [email protected]

Dear Ayad Palani,

My research group is al ready working on anti-emetic and anti-inflammatory effects of plant extracts.

We have published a number of International and National publications in this regard.

In these publications we discussed anti-inflammatory effects of plants with simplest protocols and basic discussion of suggested anti-inflammatory plant extracts (proposed mechanism of actions and possible secondary metabolites involved in this activity).

Now, I am sending you my publications in this regard.

Read these publications carefully.

If you have anymore question. Don't shy. Feel free to ask.

I always welcome you.

If your problem going to solve with my answer. Please upvote my answer.

May God help you in all your difficulties.

Regards

SALMAN AHMED

Ph.D Fellow

Article Analgesic, anti-inflammatory and antiemetic activities of Cl...

Here the second one.

Article Antiemetic and Anti-inflammatory activity of leaves and flow...

Receive third one

Article Anti-emetic and anti-inflammatory activity of fruit peel of ...

I am waiting to solve your problem if some thing left.

Regards

SALMAN AHMED

Ph.D Fellow

A good and well established in vivo model of inflammation is the air pouch method. With this method one can evaluate the anti inflammatory properties of a substance from different aspects and it gives you a good indication of how the extract exerts its anti inflammatory effects. You can measure different parameters indicating inflammation such as exudate volume, angiogenesis, inflammatory cells infiltration. You can also measure inflammatory cytokines in the inflammation site.

Look for articles with the folloeing key words;

Air pouch; carrageenan

Dear Milad,

Can you send the basic and original article of Air pouch model. There are many articles but I require the basic article.

Regards

There are many in-vitro and in-vivo models for assessing anti-inflammatory effects of compounds( phyto- extracts or synthetic ones and for acute,sub acute and chronic inflammatory conditions) given any experimental pharmacology books and research articles.

However,in order to save your time and experimental cost ,,,,...it will be better if you take a clue for which clinical inflammatory condition your plant is used (from ancient literature) you can arrive at a good and selective model for anti inflammatory activity verification.

Dear Chakravarthy,

I think at preliminary level when you have just crude plant extracts, not the purified compound. It is better to use Carrageenan induced rat paw edema model.

What do you think?

Yes, it is mostly used experimental model.But my suggestion was to be precise and caution the researcher to well study the old references of plant ( particular usage of the plant for which clinical inflammatory condition it is used exactly) so that exact models can be arrived at (Carrageenan model does not exactly reflect all clinical conditions). Secondly many plant extracts contain PHYTO-STEROIDS and show good effect in this model and such findings remained as research publication material only

Does anybody want to mention here

the list of anti-inflammatory animal models with suggested clinical conditions?

This will be more beneficial for us.

Regards

SALMAN AHMED

Ph.D Fellow

Some useful ref:

1.Animal models for the study of atypical anti-inflammatory ...

www.ncbi.nlm.nih.gov/pubmed/3962779

by B Silvestrini - ‎1986 - Animal models for the study of atypical anti-inflammatory agents. ... Clinically, they relieve the symptoms of acute inflammatory conditions, both topically and ... Available data suggest that their characteristic profile of side and therapeutic effects ...

2.Two Faces of Inflammation - Linus Pauling Institute at ...

lpi.oregonstate.edu/ss07/inflammation.html

3.Probiotic Effects on Inflammatory Bowel Disease

jn.nutrition.org/content/137/3/819S.long

4.Anti-inflammatory Disease Therapies - Toxicologic Pathology

tpx.sagepub.com/content/40/1/122.full

by S Bolton - ‎2012 - ‎

Thank you very much.

You can perform a inflammatory pain model injecting carrageenan in the rat paw and treating your animals with the extracts.

You may also like to see the attached article.

Good luck

thanks all . I appreciate your comments , I need simple in vitro assay

Dear Sir,

I have herewith attached one of our article which may help you.

Dear Palani,

You are searching for a simple in vitro assay anti-inflammatory properties for plant extracts.

Inhibition of albumin denaturation

It is a very simple. I think it not only helps you but also will solve your problem.

I am attaching a file here to solve your problem.

Read the article carefully. If you have further any query please feel free to ask.

If my given article really helps you please up vote my answer.

I am looking forward to solve your problem soon.

Regards

SALMAN AHMED

Ph.D FELLOW

thank you Salman Ahmed, that is what I look for,

If my given article really helps you please up vote my answer to encourage me.

you can made a macrophage culture and quantify inflammatory medioators as IL1 or TNF treated with your extract and stimulated with LPS.

Inflammation is a vascular phenomenon. In vivo models are much more informative an in vitro models.

The attached MS may be useful. Goodluck

Dear Salman Ahmed...

I have few queries???????????

1. What is the total volume of reaction mixture? And how much extract we have to take? And how much 1% aqueous solution of bovine albumin fraction is to be added?

2.  How much HCl we have to use and what is the final pH expected?

3. After all I am getting absorbance of control above 1 . So can i dilute all my samples and control to get the absorbance below 1? Will there be any change in inhibiton % ?

Dear Ayad Palani,

Please have a look at this link and PDF attachments.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067865/

Good luck!

The anti-inflammatory potential of plant extracts can be evaluated, using both in vitro and in vivo assays. Carrageenan induced hind paw edema model can used to carry out the in vivo anti-inflammatory activity, while for in vitro screening lipoxygenase inhibition assay can be used.

Article Evaluation of anti-inflammatory activity of selected medicin...

Determination for the IC50 for both COX 1 and COX 2 in vitro.