Milad Moloudizargari

6 Questions 29 Answers 0 Followers

Questions related from Milad Moloudizargari

Has anyone worked on exosome release?

What are the best methods to study the alterations in exosome release from a specific cell line following treatment with an agent? What if the intended agent changes the viability of the treated...

03 March 2018 1,424 8 View

Protocols for NK cytotoxicity and degranulation?

I need two detailed and reliable lab protocols one for assessing NK cytotoxicity and one for NK degranulation assay using flow cytometry. Is NK cytotoxicity done only in co-culture with K562 or...

02 February 2018 2,104 12 View

Experienced researchers familiar with flow cytometry?

Please take a look at the attached files: I am about to study 2 markers which appear in FL1 and FL2; In the unstain tube, I see 2 distinct populations one of which has weaker auto-fluorescence...

11 November 2017 6,553 7 View

What are novel adjuvants for tumor immunotherapy with the potential to be used in clinical trials?

I`m about to start my thesis on breast cancer immunotherapy which is designated to examine a nano particle-based vaccine for the delivery of tumor associated antigens to DCs in vivo. However, I...

09 September 2014 7,198 2 View

What are the possible methods for identifying and measuring Glycans in the blood?

How can we determine the level of different types of glycans in the blood?

01 January 2014 6,293 4 View

Why do the suitable voltages for my unstain and control differ completely from my treatment groups?

I`m performing a flow cytometric analysis to study apoptosis in peripheral blood mononuclear cells (PBMCs) of patients using Annexin V-FITC (FL1) and Propidium Iodide (FL2). My tubes include and...

01 January 1970 6,133 20 View