How can I estimate mannan and beta-glucan in the yeast cell walls or yeast extract?

12 December 2022 1 4K Report

I wanted to know how mannan and beta-glucan can be identified and quantified. I am looking for a simple method that we can run in the lab.

Francisco Javier Alvarez

Hi. There are protocols for their extraction and quantification by HPLC, using mannose and glucose as standards. You can find that information and look for other references in this publication: doi:10.3390/molecules19044433.

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