We have been using electroporation to transfect a hybridoma cell line with siRNA and got knockdown efficiencies between 50 and 70% which apparently is quite good for that method and that cell type. Nevertheless, I would need something more efficient and ideally in a human antibody-secreting plasmacytoma cell line. Can anyone help? Screening the literature it seems that this is not the most straightforward task... Does anyone have experience with e.g. lentiviral transfection or Crispr-Cas9?
50-70% transfection is not bad at all. If you are using siRNA, try to use Lipofectamine RNAiMAX Transfection Reagent instead of Electroporation. Crispr/Cas9 methods you need to do clonal selection (which is also going to consume some time as well) unless using the lentiviral system for Crispr/Cas9 which has antibiotic resistant gene in the system. You need to design your vector for that. For Crispr/Cas9, I am using guide RNAs and Cas9 from the company called Synthego.
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