I am looking for a method to inhibit endocytosis processes mediated by clathrin in hippocampal acute slices. I have read about different methods, the best of which seems to be: potassium depletion, chlorpromazine and monodansylcadaverine. Does anyone know or use any of that methods? May anyone recomend me a better option? Thank you very much
Dynasore inhibits dynamin, which is essential for clathrin-dependent endocytosis: http://www.ncbi.nlm.nih.gov/pubmed/18413242
Edit: typo.
Thank you Jordan! Dyansore could be a good option but the bad side is that it also inhibits other pathways where the dynamine is implicated but not the clathrine
Hi Lucas, maybe try Chlorpromazin that is often used now. Good luck!
What about inhibitors like Tyrphostin A23? It is known to inhibit clathrin mediated endocytosis. A prominent example is the inhibition of transferrin receptor internalisation.
Pitstop 2 is also reported to inhibit clathrin-mediated endocytosis. It is a small molecule inhibitor.
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