Lucas Taoro

6 Questions 11 Answers 0 Followers

Questions related from Lucas Taoro

How to introduce siRNA in human astrocyte (no primary) cultures?

Hi everyone. Does anyone try to transfect human astrocytes in culture (no primary) to introduce siRNA in order to silence gene expression? Which one would be the best option do do it effectively?...

17 October 2023 682 1 View

Which is the better composition for freezing (cryopreservation) medium for mammal cell lines?

Hi! The literature (even here in RG) does not provide a clear answer about the best composition for cryopreservative medium to freeze mammal cell lines, specially regarding to the serum component....

01 July 2019 5,707 6 View

Which antibody is recommended for immunoprecipitation of Dab1?

Hi everyone. I am interested in asses biological activity of purified reelin in cultures, so I have decided to analyze phosphorylation of Dab1 by immunoprecipitation and WB against p-Tyr residues,...

26 April 2019 8,720 3 View

How can I effectively inhibit clathrin-dependent endocytosis processes?

I am looking for a method to inhibit endocytosis processes mediated by clathrin in hippocampal acute slices. I have read about different methods, the best of which seems to be: potassium...

12 June 2016 6,985 5 View

¿How can I induce chemical LTP in hippocampal slices of rat?

I am interested in inducing chemical LTP in my model of ex-vivo slices from rat hippocampus. Does anyone know how can I do it? I men, which agonists of NMDa receptor can I use or which...

20 May 2016 2,563 6 View

Why can I see fluorescence in my control slice without Fura?

I am trying to measure calcium levels in hippocampus rat slices, specifically in CA1 layer, using membrane-permeable Fura-2. I am using control slices incubated without Fura-2 but with DMSO, the...

02 February 2016 7,543 4 View