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Questions related from Lucas Taoro
Hi everyone. Does anyone try to transfect human astrocytes in culture (no primary) to introduce siRNA in order to silence gene expression? Which one would be the best option do do it effectively?...
17 October 2023 728 1 View
Hi! The literature (even here in RG) does not provide a clear answer about the best composition for cryopreservative medium to freeze mammal cell lines, specially regarding to the serum component....
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Hi everyone. I am interested in asses biological activity of purified reelin in cultures, so I have decided to analyze phosphorylation of Dab1 by immunoprecipitation and WB against p-Tyr residues,...
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I am looking for a commercial antibody that specifically recognize phosphorylated Serine 818 in GluA1 (GluR1) AMPA receptor subunit. Thank you
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I am looking for a method to inhibit endocytosis processes mediated by clathrin in hippocampal acute slices. I have read about different methods, the best of which seems to be: potassium...
12 June 2016 7,024 5 View
I am interested in inducing chemical LTP in my model of ex-vivo slices from rat hippocampus. Does anyone know how can I do it? I men, which agonists of NMDa receptor can I use or which...
20 May 2016 2,597 6 View
I am trying to measure calcium levels in hippocampus rat slices, specifically in CA1 layer, using membrane-permeable Fura-2. I am using control slices incubated without Fura-2 but with DMSO, the...
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