How do I correct for phylogenetic nonindependence between a binary/multistate categorical variable (independent) and a continuous depedent variable?

Dear community, I am rather new to phylogenetic comparative methods and seek your advice. I want to compare a continuous trait (dependent) between species belonging to either groupA and groupB...

22 September 2020 1,618 4 View

Can you fill a quick questionnaire?

Hi there, my name is Panagiotis Koilakos and I am studying computer science in AUEB, Greece. During an HCI(Human-Computer Interaction) course, we had to select a home device and try to beautify it...

02 January 2020 4,939 3 View

How can I remove false positives using the calculated bisulfite conversion rate in a WGBS experiment?

Dear community members, We have available WGBS data for a species. We used Bismark to map the reads to the reference genome. Unmethylated lambda phage DNA was spiked-in, to estimate the bisulfite...

18 July 2018 4,081 2 View

Is there a "stress test" for fish larvae which would reveal their stress resistance without implementing violent methods (i.e. lack of oxygen)?

I need to estimate the quality of fish larvae by estimating their stress resistance OR their resistance to the lack of oxygen. However due to ethical issues I need to use non-violent methods (i.e....

27 November 2017 1,824 3 View

Is there any questionnaire or survey conducted that have measured the satisfaction of companies from a Recruitment Process Outsourcing?

I'm trying to find a questionnaire that can measure the satisfaction rate of a company according the recruitment services that a Recruitment Agency provided.

18 November 2017 9,012 3 View

Hello I would like to ask you how we can add the different sulfur compounds in hysys for the crude distillation? Thank you!

Different forms of sulfur containing compounds.

04 July 2017 9,495 9 View

Can I remove taxa from pre-existing phylogeny before running a PGLS analysis?

Hello all, I want to run a PGLS analysis. I have a phylogeny with branch lengths, but I want to run the PGLS analysis for a slightly smaller subset of taxa than the ones contained in the...

25 June 2017 7,371 0 View

How can I know all the node coordinates where pressure is calculated as also the corresponding node ID using COMSOL?

Hi,I have the square 5x5 geometry of the attached Figure. I solve a pressure distribution problem where pressure changes its values along the x-axis. I would like to know all the node coordinates...

13 September 2016 6,935 6 View

Is it possible to minimize an array of values (not just a simple function), using the genetic algorithm optimtool of Matlab?

I am conducting simulations for Genetic Algorithm using optimtool('ga') of Matlab. I would like to minimize the difference of two arrays so that I can fix the valueof a parameter which is the...

09 May 2016 4,987 4 View

Safe concenctration of Guinidine hydrochloride in cell cultures?

Hello, I need to use guinidine hydrochloride to clear any aggregates of apoA-I in my solution and then treat cells with it. What is a safe final concentration of GndHCL in the cell cultures?

26 March 2016 3,983 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View