Hi Mr. Hani Antoun,

Thanks for replying.

I used air dried method as pretreatment before i made serial dilution. and i am using Mannitol soy agar & Tap water yeast extract agar as isolation media.

but i am still confused, which microbes that I should purificate, Sir hehe

Best regards,

Gema

In general, the actinomycetes colonies on agar plates are characterized by:

- It is not easy to pick them up by the loop because they embedded into the agar except the rough surface (In most species). On Contrary, the colonies of fungi and bacteria are so easy to pick them up by the loop. In addition, the fungal mycelium is long and visible by naked eye.

- Actinomycetes form not spread small colonies of chalk touch.

Best wishes 

Dear, The attached file is clearly shows the morphology of actinomycetes colonies (our special research work). I hope it clears your confusion.

Actinomycetes have chalky texture. They have aerial mycelium with different color. Also, they have background with different color. Sometimes there are  colored pigments in the agar. 

You can also add to the medium, cycloheximid 0.050ml/liter and nalidixic acid 0.010ml/liter.

Actinomycete colonies are powdery,half embedded in solid media, have earthy smell,sometimes produce pigments.