Hello to all and thanks for your help!
I have extracted RNA using standard protocol of Trizol reagent and Phenol chloroform extraction, followed by DNAse treatment and purification .
How can I test the quality of the RNA before starting with the standard curve?
I get very low RNA concentration ( mean 100 ng/ul) and if I need to use 1 Ug to prepare cDNA ... I have not too many sample to run.
Which is the minimum RNA quantity that I should run to see something? What am I expected to see if I run the RNA in a regular agarose gel using TAE buffer... without previous denaturation?
I much appreciate your help!
Thanks :)