Hello to all and thanks for your help!

I have extracted  RNA using standard protocol of Trizol reagent and Phenol chloroform extraction, followed by DNAse treatment and purification . 

How can I test the quality of the RNA before starting with the standard curve? 

I get very low RNA concentration (  mean 100 ng/ul) and  if I need to use 1 Ug  to prepare cDNA ... I have not too many sample to run.

Which is the minimum RNA quantity that I should run to see something? What am I expected to see if I run the RNA in a regular agarose gel using TAE buffer... without previous denaturation?

I much appreciate your help!

Thanks :)

Similar questions and discussions