I tried a method using CTAB buffer.. lets see if PCR works.. DNA concentration is about 10-12 ng/microliter... this shlud work but I get no results in previous extraction and get same concentration.
I would like to get a DNA sample which I know works, to use as a positive control... in case someone here has I will be happy to get in contact!
Waqar: I'm extracting from the whole body... may be you suggest to take a subsample?
Thanks Waqar! I actually succeded to extract DNA with CTAB buffer, using a KIT for Plant DNA extraction... and it worked nicely. PCR work fine as well... but sequencing didnt work:) I will continue trying... sometimes things does not work out as well as it appears in the published articles :)