10 Questions 19 Answers 0 Followers
Questions related from Suleyman Bozkurt
Hello Everyone, I am trying to measure mitochondrial membrane potential (MMP) with specific mitochondrial dyes name as "MitoTracker Red CMXRos". I am using CCCP to reduce MMP to induce...
06 June 2019 1,032 5 View
Dear RG community, I treated my cells with CCCP and Antimycin/oligomycin mix at different time points and looked for a protein. In the literature, there is not similar image that I found in my...
05 May 2019 9,638 11 View
Dear Community, I am master student and working with suspension cells. For western blot analysis, I am plating cells right away after collection and induce incubation with any drugs that I am...
07 July 2018 446 3 View
Dear RG community, I have a substance that I want to use it in the cell culture (in vitro). I am using flow cytometry for mitchondrial dynamics and western blotting for mitophagy/autophagy. But I...
05 May 2018 2,526 3 View
Dear all, I have been doing qPCR (after reverse transcription of my RNAs) some time now. I am using sybr method. Ive tried 8 cDNA. Initial cDNA amount is not equal in each sample. Because my RNAs...
11 November 2017 6,920 7 View
Hello, I've been doing flow cytometry nearly 6 months now. But still I did not figure it out a dye we are using. It is a specific dye called "MitoTracker Red CMXRos". It is special dye to...
10 October 2017 1,001 12 View
Dear all, My lab was isolated leukocytes and stored at -20C. And now I am trying to isolate DNA from it by using Zymo DNA isolation minipreb kit. I encountered several problems, first I could not...
06 June 2017 1,086 3 View
Hello, I am trying to do Methlation specific PCR and for that I have to design primers for it but when I search my gene of interest in ensembl, it shows two adjacent promoters, part of one of this...
12 December 2016 5,343 4 View
Dear friends and colleague, I just want to work on cancer especially (triple negative breast cancer) early diagnosis and detection. So that I just want to analyize cancer data for finding markers....
06 June 2016 8,137 4 View
Dear Colleagues, Can you check my gel result? It is about double digestion of plasmid. But some fragments are seen bad can you say something about that ? Are they contaminated ? or were enzymes...
03 March 2015 320 4 View
