How to determine the range of concentrations for use a ruthenium compound in a toxicity test MTT, references talk about ranges (that for example) ranging from 300 uM to 100 nM , do not speak of the basis to determine about this. thanks for your attention.
Well just make like 7-8 conc in decreasing order (try like 1000, 100 10 1uM and 100 nM 10nM 1nM) or something like that. Its your choice and until you get your results you can be sure. Also repeating of results is advisable and then you can change those conc.
Selection has to be random if it is an unknown chemical. The suggestion from Nikola makes perfect sense in the case of unknown compounds.
However, if you have designed and synthesized the chemical compound using Structure-activity relationship, then you can use IC50 of parent compound (if reported) as a guidance to decide the concentrations to be used.
Alternatively, I personally prefer to search for the closest molecule in chemical libraries with a known IC50 value. I use the IC50 of that closest molecule (or closest 2-3 molecules) to decide the concentrations to be tested. If you get one of these close molecule, then I would recomment using 2-3 concentration above and 2-3 concentration below the IC50 of that compound.
IMHO the best way is to use a logarithmic range of concentrations, like 500, 200, 100, 50, 20, 10, 5, 2, 1... This covers a wide range of concentrations with not too many points.
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