Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

What is the best way to extract Spike protein from whole cell lysates for ELISA ?

Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...

17 September 2020 7,265 4 View

Does anyone have a reliable protocol to assess cell viability in commercially available GelMA (Gelatin methacryloyl) ?

Hello all, I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing...

13 September 2020 9,086 1 View

No title

They are software's that are used mainly in the discipline of psychology

05 August 2020 9,423 3 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

Which value of volume fraction I can to choose for to identify free surface in an air-water simulation of open channel?

In air-water multiphase flow CFD simulation's it is common to use a VOF = 0.5 to track or identify the position of interface or free surface. I would like to know why this criteria is used and if...

12 July 2020 7,793 9 View

How to decide if to get 1 or various P value? regarding an composting process through time and treatments?

I have a research investigation that I got the results, I applied 4 different composting processes to vegetable waste (manure, manure+Effective Microorganisms, Effective Microorganisms and a...

14 June 2020 2,373 2 View

What is a CFD and other question?

To me, a CFD is solve a fluid mechanics problem using mathematical approaches with the help of a computer. In other words, it is like a marriage between a mathematical model and numerical methods...

04 May 2020 2,811 25 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View