How can I convert U/ml to µg/ml ?

30 November 2018 1 1K Report

Hello everyone,

Soon I have to prepare 20 ml of a lysozyme solution and I need 10µg/ml DNAse 1, the concentration of DNAse 1, which I have is 2 U/ml. How can I convert this?

many thanks :)

Derya Erguen

Frida Gorreja thank your very much. That was really helpful.

Badges
Science topic

More Derya Erguen's questions See All

T7•Tag® Affinity Purification Kit Problem ?

Hi! I have usedT7•Tag® Affinity Purification Kit (Novagen. It says it can purified up to 300ug protein but it did not work efficiently for my purification. Has anyone used it and faced any...

28 February 2023 6,133 0 View

Why can't I print tissue scaffolds properly?

Hello, I've been working with a 3D SLA printer to produce a bone tissue scaffold and trying different parameters, both the ceramic slurry and the 3D printer. However, I haven't achieved successful...

07 February 2023 3,169 6 View

How can I write multigroup SEM syntax in JASP?

Hi everyone, I have conducted multigroup sem in JASP. I got measurement invariance test results that I expected. Now, I wonder to know how I can write the syntax showing constrained path to be...

28 December 2022 2,457 2 View

Why are the DNA bands not obtained on agarose gel before DNase I treatment ?

I performed RNA isolation from B. subtilis (lysozyme combined with Trizol reagent method) and RNA samples were treated with DNase I. I had samples for running an agorose gel both before and after...

01 September 2022 796 1 View

What are the methods and techniques used for voice training in traditional Arabic music?

I ‘m studying to develop a voice training method to be used in Traditional Turkish Music Education. So I need the approaches of different cultures that use makam music.

10 April 2020 5,969 0 View

How does downregulation of T cell receptor and CD8 molecules work on a molecular level?

Hello everyone, I have a question about my research topic. I used a tyrosine kinase inhibitor (Dasatinib) to improve detection of CD8+ cells, according to a few publications (Wooldridge et al....

29 March 2020 8,081 2 View

Can you help me find the specimen of Anotylus sexualis?

Hi everybody, Does anyone have the specimen/s of Anotylus sexualis (Eppelsheim, 1892) (Staphylinidae: Oxytelinae) on own collection? Or, do you know someone else have the specimen on his/her...

21 December 2019 3,251 3 View

What are the negative effects of social life to students?

I have to write an cause and effect essay about too much attention to social life. I have to mention about unsuccesful students who are showing too much attention to their social life. I looked...

19 December 2017 357 9 View

Can anyone explain what’s the procedure for people who present psychiatric decompensation during a prison sentence in YOUR country?

Hi, I’m carrying out my master thesis on people who were sentenced to serve time in prison and who, during incarceration, present psychiatric decompensation. I’m interested in the official...

26 November 2015 6,365 10 View

How can I calculate the improper integral using Matlab?

Dear Prof., We have an integral in which we have singularity. We try to use Matlab for numerical calculation. However, We have not found the correct way to calculate the integral. We use trapz or...

26 November 2015 730 8 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

Can I use DNase I and RNase R in the same moment?

Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous...

02 June 2024 9,544 0 View

DNA foot-printing buffer components? What are their roles?

Hi, I am learning to perform footprinting experiment. In the protocol I have there are the following components of the reaction buffer: HEPES buffer (7.8 pH), glycerol, BSA, DTT, KCl, MgCl2,...

28 May 2024 1,317 0 View

Can lysozyme also interact with dextran and agarose?

Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...

25 May 2024 5,979 3 View

Why can't i download my article to my page?

Use of a maltodextrin-based feed with a lysozyme product to ... Kindly add this article to my profile. Thank you very much Regina

16 April 2024 9,905 0 View

Will adding DNAse to lysate in phosphate buffer affect its activity?

Hello, everybody. I am looking to add a DNAse treatment step to my bacterial lysis (gram-negative) procedure for crude enzyme extraction because my lysate always ends up being too viscous. We...

14 April 2024 280 2 View

Does DNase I have lower activity on supercoiled DNA?

Hello everyone, I am working on the production of IVT mRNA. After transcription, the remaining DNA templates are digested by DNase I. I have tested transcription with uncut DNA (mostly...

10 April 2024 1,362 2 View

Any advice on how to do cell lysis using osmotic shock?

Hello everyone, I am supervising a master thesis student whose goal is to compare different lysis methods for recombinant protein purification. We are working with E. coli pellet and he is...

08 April 2024 6,240 7 View