How does downregulation of T cell receptor and CD8 molecules work on a molecular level?

Hello everyone, I have a question about my research topic. I used a tyrosine kinase inhibitor (Dasatinib) to improve detection of CD8+ cells, according to a few publications (Wooldridge et al....

29 March 2020 7,961 2 View

What are the negative effects of social life to students?

I have to write an cause and effect essay about too much attention to social life. I have to mention about unsuccesful students who are showing too much attention to their social life. I looked...

19 December 2017 229 9 View

How can I change number of my kernel density estimation values without changing 'std'?

Hello eveyone, I have mean value is 60, and standard deviation is 10. I'm generating 50 and 800 samples to see kernel. From kernel density estimation in Matlab, I'm getting high 'std' like 20ish...

25 June 2017 8,038 1 View

What software is being used for retention time prediction?

I am looking for the tools to predict peptide retention time for LC-MS.

10 April 2014 9,604 4 View

LC-MSSim installation problem. How to adopt the makefile and execute make?

I am following the installation instructions at this website: http://lcms-sim.sourceforge.net/ However I am stuck in the 3rd instruction. When i open the makefile, it says: # You need to set...

07 April 2014 5,329 2 View

What is the optimal amplicon size for qChIP?

I have sonicated ChIP DNA which smears btw 200-1000 bp. I read that the amplicon size for qChIP should be btw 200-350 bp; however 50-150 bp is recommended for qPCR. Does anyone has experience or...

16 February 2014 9,432 3 View

How to start modelling biological systems?

I am supposed to model EGFR trafficking. I will use matlab, maybe simbiology. But I don't know where to start. I did not do something like that before. I was good at differential equations years...

02 December 2013 7,143 3 View

FACS analysis for B cell survival test ( PI and 7-ADD)

I need help to analyze the FACs results, which were performed on B cells to assess the cell survivability. I used PI and 7-ADD dyes and I saw two distinct cell populations in the SSC vs. FS blot....

28 May 2013 4,164 4 View

Why am I getting signal in my mock cells but not in the negative control?

I have been having troubles with qPCR which frequently give signals in the mock samples. My experimental set up consists of samples that were infected with virus and mock samples which were not...

10 February 2013 1,916 11 View

Nucleofection- large size plasmid delivery- suspension cells- Lonza Amaxa 4D-X system

Working with CRISPR-Cas editing in suspension cells. I need a successful delivery of plasmids to suspension cells using nucleofection method ( Lonza Amaxa 4D-X system). The problem is this...

01 January 1970 1,375 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

No title

01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View