For real time pcr, does it matter which part of the gene region the primer expresses?

I want to determine the amount of expression by performing qPCR of a bacterial gene region. For this, I compared some primers found in the literature. Some of these primers targeted the starting...

09 January 2021 9,475 3 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

NMR metabolomics identification software?

Hi everybody, Does anybody know an alternative way to identify and quantify the 1D-H NMR spectrum for metabolomics studies other than Chenomx? We have no budget for Chenomx and I need a freeware...

29 August 2020 2,877 3 View

GC-MS Column for Metabolomics Studies?

28 June 2020 8,404 3 View

How does downregulation of T cell receptor and CD8 molecules work on a molecular level?

Hello everyone, I have a question about my research topic. I used a tyrosine kinase inhibitor (Dasatinib) to improve detection of CD8+ cells, according to a few publications (Wooldridge et al....

29 March 2020 7,961 2 View

Can you help me find the specimen of Anotylus sexualis?

Hi everybody, Does anyone have the specimen/s of Anotylus sexualis (Eppelsheim, 1892) (Staphylinidae: Oxytelinae) on own collection? Or, do you know someone else have the specimen on his/her...

21 December 2019 3,122 3 View

How to do Bland Altman Plot in measurement pairs in SPSS or Excel?

Hi all, Although I watched all the videos about Bland Altman plot in SPSS, I could not find any information regarding how to use this statistics in multiple replicates for each method. Hope...

13 September 2018 9,359 14 View

What are the negative effects of social life to students?

I have to write an cause and effect essay about too much attention to social life. I have to mention about unsuccesful students who are showing too much attention to their social life. I looked...

19 December 2017 229 9 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

How to use bag of words for action prediction?

Hi everyone, I'm working on an action prediction (i.e., early recognition) using Bag-of-Words. I know how to use BOW, but regarding action prediction, there is a subtle problem. In the...

19 February 2021 1,442 4 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Discuss the extent to which morality in African traditional societies can be classified under the Natural Law theory of morality?

How can one classified African traditional societies under natural law

18 February 2021 7,334 8 View