Hi!
I have usedT7•Tag® Affinity Purification Kit (Novagen. It says it can purified up to 300ug protein but it did not work efficiently for my purification. Has anyone used it and faced any problem? Thanks.
i want to know; that how to proceed with the mentioned data set to capture the long run and short run association in panel data, thank you
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Dear: I would like to prepare a sterile solution for injection of animals under my experiment with 1,3-ᴅ-glucan. but it was recorded that, steam autoclave affects the criteria of the extract. So,...
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Why mRNA and protein levels do not correlate?
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I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
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List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
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Purification of gst tagged protein
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I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
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What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
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