I found white particles or sample precipitates in the sephadex gel already used 4-5 times. How can I remove it. I have read to use 0.2 M NaOH or non-ionic detergent for cleaning. But I couldn't find its detail procedure i.e. for how much time I should keep gel in the detergent or how many times I should wash it? Please tell me the detail procedure.
Thank you
Hi
Please see the description in the link:
http://www.gelifesciences.co.jp/tech_support/manual/pdf/56119096ac.pdf
Page 7
Greatings
Hi,
Try autoclaving at 120 degrees for 10 min and then wash with 2 column volumes of 0.2 M NaOH (5 min) and then reequilibrate with the gel with 2-3 column volumes of buffer. Store the used gel in 2-8 degrees in 20% ethanol.
I separate condensed tannins in 750 ml wine samples using Sephadex LH_20 by using a gradient of Methanol (M) and Acetone (A).
I equilibrate the column with 50 % Methanol, then seperate
60M,
75M,
90M,
80M:10A,
65M:20A,
40M 30A
60A (Acetone elutes the condensed tannins I want)
How do I clean my column - I have been told that DMF or DMSO might work; what what I clean using the starting solvent? Would that do?
Assistance would be appreciated - thank you.
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