How can I check if the cloned gene is in the frame?

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Marcin Nowicki

First you need to sequence the constructs, using the pET-specific primers. Then, yes, you do work with the resultant sequences to ensure in-frame status.

Shanice Chiang

Thanks DR. Nowicki

Pranali Deore

Try doing cloning in Nco-1 or nde-1 , these sites at 5' end gives ATG so that your construct is in frame.