Hi Kristy,

It is a bit hard to say what is the maximum of cells as it is more a question of volume. Mice (C57BL/6) are ok to receive 200uL of sterile PBS containing lymphocytes. If it helps I transferred 10 millions of total CD19+ into RAG-/- without having any problems. It is important that there are no air bubbles. It also helps to filter the cells before injection in order not to have cell debris.

I hope it helps. Good luck,

Andreia