After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How can I set to thin film on the substrate in ZEMAX?

My research is about 3D-printing add nanoparticles attach to copper. So I'm trying to use ZEMAX to calculate absorption, someone knows how to set the thin film on ZEMAX? Have any suggestions or...

26 November 2020 7,200 1 View

Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

A genome wide CRISPR/Cas9 screen for drug resistance in cancer cells?

03 November 2019 7,662 2 View

How to identify RAMAN spectrum especially for plant ?

How can I obtained plant RAMAN spectrum database for free ? or is there any useful reference can be used for the analysis of plant RAMAN spectrum ?

20 October 2019 9,268 1 View

What kinds of single indicators are appropriate, when we calculate multifunctionality?

A (wide accepted) view is that, when we try to address on the biodiversity-ecosystem function relationships, the multifunctionality index should be calculated by a set of activity indicators,...

02 September 2019 8,370 2 View

How to choose solvent C18 column for separating polar compounds?

I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08,...

06 August 2019 3,572 2 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

How the type of nanoclay affecting composite's mechanical properties?

Please forgive me for without any introduction, I will try to make it short. I am understand that nanoclays can be classified as T:O (tetrahedral: octahedral ) or T:OT layer stacking . I also...

17 June 2019 7,881 3 View

How to present the complete results of stepwise regression?

The common form is the presentation of regression equation, with the coefficients of determination and p value included in the brackets. But we know that the beta coefficients and sum of squares...

02 March 2019 6,064 6 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View

In Method prepreation, what is the meaning of (Fixed at), and the meaning of (mol% of somthing) and how to find the volume?

I need an explanation of this sentence: Firstly, SnCl4.5H2O with fixed at 0, 1, 3, and 5 mol% of TiO2 and Titanium (IV) isoproxide (TTIP) with fixed at 10 ml were mixed into 150 ml of ethanol...

10 February 2021 3,291 2 View

How can we quantify martensite in Ferrous and Titanium alloys?

How can we quantify metastable phases like martensite in Ferrous and Titanium alloys, in relation to their stable counterpart For example in EBSD, XRD, Optical? Eg, Martensite and Ferrite maps.

09 February 2021 7,572 3 View

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View