Shanice Chiang

10 Questions 16 Answers 0 Followers

Questions related from Shanice Chiang

Can anyone recommend a website that can predict RNase E cutting site?

Do you know of any website which would predict RNAse E cutting site? or how to predict it? We are having problems expressing a foreign mRNA in E.coli and one possibility was that the mRNA was...

18 October 2014 10,002 3 View

how to tell if protein induction works?

I am not sure if my induction have worked. Is it possible to do a RNA miniprep. IF RNA successful extracted- means induction has worked.

14 October 2014 3,160 0 View

Would I be able to see a difference between a induced and uninduced empty pET vector?

Hi people, I am having a lot of difficulties in my protein expression. I am wondering if my induction has failed to work? Too bad, i dont have any positive control. So i am using the empty...

13 October 2014 7,498 8 View

D-lactose monohydrate versus alpha lactose monohydrate for use in autoinduction media

I need lactose for my autoinduction media for E.coli. Just wondering if I could use the D-lactose monohydrate because I can't find any alpha-lactose monohydrate in my lab. Are they the same?

09 September 2014 6,179 7 View

Can anyone help with induction in E. coli?

Dramatic induction was observed, but no desired protein band was observed. I run an empty pET as a control. There doesnt seem to be difference with the empty pET and pET with insert. I have no...

11 August 2014 3,833 12 View

How does one extract inclusion bodies from E. coli?

what is the most effective method here

02 August 2014 7,311 2 View

Can you give me some advice about my PCR on cDNA?

Dear all, I tried to extract RNA from different rice plant tissues and then convert it to cDNA. I then amplified the cDNA with different set of primers. Only one of them showed the expected size....

22 July 2014 3,133 10 View

How can I check if the cloned gene is in the frame?

Hi all, I have just cloned my gene into pET and would like to check if my gene is in frame. Can I just translate my sequence and then do a protein blast is that a proper way of doing it?

18 July 2014 1,476 3 View

Could anyone help me with my protein expression protocol?

Protein Expression in Prokayotic (Rosetta (DE3) E. coli) (1) Transformant Rosetta (DE3) cells with recombinant pET28(+) were grown on LB/Kanamycin/Chloramphenicol agar plates overnight. (2)...

14 July 2014 8,015 5 View

Failed cloning over and over again

Dear All, I need some help. I have been trying to clone my gene into pET28+ for months but the results is always disappointing. My brief protocols are as per follow: 1. PCR on my gene of...

07 July 2014 8,386 17 View