Hello, I am running an Optiprep iodixanol gradient density to isolate my exosomes. With each run I also have a control which I exact 12 fractions and from the protocol it said I need to calculate the density of each fraction so I will know in which might be my exosomes.
However, although it might be quite straight forward, I am having difficulties in calculating the density of each fraction. I have been given the coefficient of extinction 320 L g-1 cm-1 and the wavelength 244nm. And with that I dilute each franction to 1:10000 and read the absorbency. However, I do not know how to get to the density from there. Any suggestion?
Thanks