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Questions related from David Roig-Carles
I hope everyone is getting on with their research. I am about to isolate extracellular vesicles (EVs) using frozen human plasma because I do not have access to fresh samples. Thus, I was wondering...
09 August 2017 231 3 View
Hi, I am isolating exosomes and one of the method I am testing is the comercial kit of EXIQON. So far I have found it gives a better yield than ultracentrifugation and same size population...
25 May 2016 595 7 View
hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...
18 May 2016 1,437 3 View
Hello, I am running an Optiprep iodixanol gradient density to isolate my exosomes. With each run I also have a control which I exact 12 fractions and from the protocol it said I need to calculate...
04 February 2016 4,278 2 View
Hi, I am starting my PhD and want to isolate exosome from my human brain endothelial cells for transcriptomic analysis (microarray and qPCR). I have been reading about different isolation methods...
18 November 2015 2,235 14 View