David Roig-Carles

5 Questions 13 Answers 0 Followers

Questions related from David Roig-Carles

Does anyone have experience isolating exosomes (EVs) from frozen human plasma using ultracentrifugation

I hope everyone is getting on with their research. I am about to isolate extracellular vesicles (EVs) using frozen human plasma because I do not have access to fresh samples. Thus, I was wondering...

09 August 2017 273 3 View

~What is your opinion about EXIQON exosome isolation kit?

Hi, I am isolating exosomes and one of the method I am testing is the comercial kit of EXIQON. So far I have found it gives a better yield than ultracentrifugation and same size population...

25 May 2016 657 7 View

Do homozygous Cre-expressing mice show any problem?

hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...

18 May 2016 1,503 3 View

How can I calculate the iodixanol density when I am running an Optiprep gradient density for exosome isolation?

Hello, I am running an Optiprep iodixanol gradient density to isolate my exosomes. With each run I also have a control which I exact 12 fractions and from the protocol it said I need to calculate...

04 February 2016 4,349 2 View

How much starting material do I need for exosome isolation?

Hi, I am starting my PhD and want to isolate exosome from my human brain endothelial cells for transcriptomic analysis (microarray and qPCR). I have been reading about different isolation methods...

18 November 2015 2,286 14 View