Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Would there be any difference in transfection efficiency of plasmid eluted from mini and maxi prep?

24 September 2020 8,198 3 View

Dispersion of Graphene?

--

14 September 2020 8,364 5 View

How to reduce STL file size for simulation study?

I have created a lattice structure using Autodesk Netfabb and saved it as a .stl file. I want to export it as .iges to ANSYS to conduct FEA but the file size is too big and it crashes. When I try...

27 March 2020 4,136 3 View

How to input a particular value of frequency using ABAQUS FEM software?

Dear fellow scientists, I am working on a project which is in correlation with acoustic imaging using ABAQUS. I am having trouble inputting a particular value of...

05 August 2019 8,865 8 View

ShRNA (bitter taste receptor sub type konkdown in adult cardiomyocytes cells, its not reached successfully transfected based on fluorescence ?

ShRNA (bitter taste receptor sub type konkdown in adult cardiomyocytes cells, its not reached successfully transfected based on fluorescence expression and mRNA quantification by QPCR? Give some...

12 November 2018 1,518 2 View

Band broadening in sequencing gels: How to avoid?

Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...

03 November 2018 4,008 8 View

Do Protein A/G agarose beads nonspecifically bind to DNA?

Hi all, I am performing a Co-IP experiment where would like to check the binding of my protein to the DNA of interest. I kept a control where I incubated the DNA and antibody only with the...

08 May 2018 6,193 5 View

Does TON (turnover number) value have any units?

Does TON in catalysis have any units? I have seen overlap of TOF and TON (TOF=TON/t), but papers seem to be using s-1 for both. I will be grateful if anybody can shed light on a proper...

06 October 2016 6,305 4 View

How can I dissolve hydrophobic compounds in DMEM media?

07 December 2015 954 5 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View