8 Questions 20 Answers 0 Followers
Questions related from Subramaniyam Ravichandran
Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...
04 November 2018 4,107 8 View
Hi all, I am performing a Co-IP experiment where would like to check the binding of my protein to the DNA of interest. I kept a control where I incubated the DNA and antibody only with the...
09 May 2018 6,300 5 View
I would like to calculate the concentration of T7 polymerase in the enzyme from NEB. Can anyone tell me how I can do this? Thanks in advance.
22 April 2018 6,311 3 View
Does TON in catalysis have any units? I have seen overlap of TOF and TON (TOF=TON/t), but papers seem to be using s-1 for both. I will be grateful if anybody can shed light on a proper...
07 October 2016 6,398 4 View
Hello all, I have taken the 1D proton NMR spectrum of my DNA sample in 90% H2O and 10 % D2O. I have also taken the spectrum of my sample with the ligand (dissolved in deuterated acetonitrile)...
13 April 2016 6,430 3 View
I am trying to generate cDNA from MCF-7 cells. So far, I have been able to extract total RNA using a combination of Trizol and Qiagen RNEasy kit. My A260/280 values are all above 2.0 and the...
30 July 2015 8,574 1 View
I am trying to find a protocol for performing CD melting experiments on oligonucleotides to analyze their Tm values. All papers mention that they collect the ellipticity at a specific wavelength...
23 February 2015 5,906 4 View
I have a fwd primer with 37% GC content and the rev primer with 61% GC content. The Tm of the fwd primer is 55.6 C and that of the rev is 58.2 C. Upon performing gradient PCR, I saw bands at 50C...
25 December 2014 3,614 8 View
