LOD is the minimum amount (concentration) of an analyte (a small peak) which you can differentiate from you blank. LOQ is usually 3 times the concentration of LOD. eg. If your LOD is say around 5 ng/mL, you can consider 15-20 ng/mL as your LOQ. Hope it helps.
Hi,
this article explain very well the approaches for calculation of LOD and LOQ in HPLC analysis.
Hope it helps you.
http://www.sciencedirect.com/science/article/pii/S1570023208007265
http://www.sciencedirect.com/science/article/pii/S1570023208007265
can any one send full text paper of above url
normally the LOD is 3 time to signal to noise ratio, and LOQ is 10 times to signal to noise ratio
For determining the real minimum amount for each of the three sugar I can quantify may be interesting trying to analyze by HPLC more and more honey dilute solutions. I would also like trying to make a surrogate honey (I suggest...!) where I increase (by addition, if it works) two of the three sugars (e.g. fructore and sucrose) and make the third (e.g.glucose) minimized at the lowest relative levels. Then I would like trying to analyze this surrogate honey and see if the lowest levels of the third sugar I can detect, in a honey matrix. This has to be done for each of the three sugars.
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