Citric acid, malic acid and lactic acid by HPLC

More Salma Salman's questions See All

Reasons for ticking sound from foreline oil pump in GCMS?

regular ticking sound from GCMS foreline oil pump, the sound incrase during injection

09 October 2017 9,623 2 View

Dear all , i need answer for those question :How can you prepare and seprate nanobiomaterials, prepare of surface modification of metal particles ?

i need to general methodolgies

31 December 2015 3,702 3 View

Can anyone suggest a suitable solvent for GCMS?

What is the most preferred solvent for dissolving a sample prior to injection on GC/MSD especially for pesticide analysis?

06 July 2014 4,916 29 View

Inlet in GCMS

what's the function of inlet in GCMS , and what's the advantages of Multi mode inlet over other types ?

06 July 2014 9,148 1 View

How can I calculate LOD and LOQ for sugars in honey (fructose, Glucose ,Sucrose) when they are measured by HPLC , RI detector, NH2 Column?

01 February 2014 9,299 8 View

What is the best LC - Column for carbohydrate analysis?

If I have the choice between 2 amino columns for carbohydrate analysis: Luna amino column from phenomenex and Pinnacle from restek which one should I choose?

10 November 2013 3,452 6 View

Does anyone have experience with amino acid determination of amino acids by GC-MS using a EZ-Fast kit?

Does anyone have experience with amino acid determination of amino acids by GC-MS using a EZ-Fast kit and its advantage over an amino acid analyzer.

08 September 2013 4,639 4 View

How can the lactose content in milk be determined using HPLC?

Looking for a method to do this.

11 December 2012 7,093 8 View

Rezex column

does any one use rezex column for carbohydrate analysis by HPLC?

09 October 2012 10,030 0 View

Rezex column for carbohydrates analysis

Does any one use rezex column for carbohydrates which is an ion exchange column? Is it more accurate than amino column for sugar analysis?

09 October 2012 6,257 13 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Alok Nahata Popular answer

This file deals with the organic acids found in foods and HPLC method to evaluate them. This is a nice presentation with all the experimental details.

Gud Luck

Kyo Bin Kang

I guess HILIC column should be used for them

Małgorzata Jaworska

Some vendors offer dedicated columns for Organic Acid separation (OA) e.g. Dionex - Acclaim Organic Acid Column; Grace Davison - Prevail Organic Acid Column; Phenomenex - Rezex ROA-Organic Acid Column. The separation is based on ion-exclusion mechanism with use of dilluted sulphuric acid or low conc. phosphate buffer of low pH as a mobile phase. UV detection at 200-210 nm is sufficient. Usually you can find some guide or application on wendor's website. I've worked with Prevail OA Column with success.

Alok Nahata

Pl find attached some files which directly answer your question

This pdf has details for analysis of organic acid standards using HPLC.

Robin Joshi

We have published on organic acid by HPLC. Please find attachment.

Article Cold-Adapted and Rhizosphere-Competent Strain of Rahnella sp...