I need help to understand how to calculate expression level of microRNA as I know the standard method is to calculate gold change through ΔΔCt = (CtmiRNA − Ct refrence RNA) patients − (CtmiRNA − Ct refrence RNA) controls. Equation but I found in some papers gave the expression level of microRNA as numbers as I know the expression levels calculated as fold changes compared to control group so where did these numbers come from maybe I'm missing some thing here. And how can I calculate AUC for their Levels