Farruk Lutful Kabir

3 Questions 20 Answers 0 Followers

Questions related from Farruk Lutful Kabir

What would be the optimum ratio of two reporter expression vectors (for gene of interest) when co-transfected in mammalian cells?

I am working with miRNA expression in canine cancer cell lines. This particular question refers to my reporter assays to test the miRNA and 3'-UTR target sequence binding in cancer cells. I am...

08 August 2014 5,825 6 View

QPCR data analysis: what is the correct way to represent the results?

I am doing qPCR using SYBR Green fluorescent dye. I am looking at gene expression of some biomarkers in cancer cells. I have GAPDH or L37 as reference gene and also a normal cell line related to...

08 August 2014 9,304 4 View

What is the best percentage protein gel for a 16 KDa protein sample using Western blot?

I am working with a 16-KDa protein on 4-20% gradient gel in my Western. I tried both monoclonal and polyclonal antibodies. Every time I am getting sharp band with a molecular weight matching ~20...

07 July 2014 8,827 13 View