Hello Saud Alyafei. It will be best to make serial dilutions of your suspension (1/100 - 1/10,000), and plate an amount (10-100 ul) on agar plates. The next day, you can literally count the colonies per plate, and use a formula of CFU counting, taken into consideration of the dilution factor and the volume of suspensions. This will be more accurate compared to absorbance readings, since OD600 will also read the non-bacterial contents of your suspension, eg. waste materials.

Hello Saud Alyafei. It will be best to make serial dilutions of your suspension (1/100 - 1/10,000), and plate an amount (10-100 ul) on agar plates. The next day, you can literally count the colonies per plate, and use a formula of CFU counting, taken into consideration of the dilution factor and the volume of suspensions. This will be more accurate compared to absorbance readings, since OD600 will also read the non-bacterial contents of your suspension, eg. waste materials.

Thank you very much Nurul 

Hello Mr. Saud;

there is a general and simple way to calculate your bacteria :

average of bacteria from two plate X dilution factor / wt of sample.

thanks

Ismail

Oman

Thank you Ismail