Ehsan Razmara

2 Questions 13 Answers 0 Followers

Questions related from Ehsan Razmara

Problem with huge mutation rate in Cloning ?

We have amplified a slightly large segment of K562 cDNA -2271bp- in E.Coli TOP10. At first, we’ve used of MaxTaq Enzyme (Vivantis Com.), but the mutation rate was so great and we found 5...

11 September 2017 4,904 5 View

What's the best source to check the gene expression in Ankylosing spondylitis ,PBMC , macrophage or monocyte ?

I would like to check the gene expression in the AS patients, but I don't know, I must measuring these genes in PBMC, macrophage or monocytes? Meanwhile, my mutant gene doesn't have any expression...

04 January 2017 8,651 2 View