How can fungal spores be standardized to the required test concentration from the crude suspension

26 August 2016 1 4K Report

The spores are usually harvested in the crude form whether from the culture plate, cellophane or broth. How can the different concentrations required in an experiment be obtained from the crude spore suspension. Is it same as in serial dilution?

Rabiul Islam

Dear Fredrick Umaru,

When serial dilution or create various concentration of fungus spore from Culture of same plate or broth media, thats time plating any type media result may be varioation but it's something.

Badges
Science topic

More Fredrick Umaru's questions See All

The economic and operational impact of theft and vandalism on affordable housing developments sites in Kenya?

Effectiveness of Physical Security Measures in Enhancing Safety on Construction Sites in Kenya

12 April 2024 1,462 1 View

Beyond the headlines, why is it that state-owned enterprises in Kenya are not self sustaining?

Beyond the headlines, why is it that state-owned enterprises in Kenya are not self sustaining? Is privatization the solution? And if privatization is the solution: what the best way to deal with...

18 January 2024 3,408 0 View

How to develop a web-based Public Participation Geographic Information Systems (PPGIS) platform???

I want to know how to create PPGIS web-site platform and how it is used to collected data and create maps.

02 December 2023 2,092 2 View

What is the best quality of subsurface geological reservoirs for large-scale Underground Hydrogen Storage (UHS)?

I desire to know the best type and quality of geological reservoirs used to store hydrogen gas underground. Methods used to assess the viability of these rocks to store hydrogen gas.

02 October 2023 4,206 4 View

How is Q-Slope Classification System applied to determining and analyze slope stability in opencast mines for sustainable mining and development?

I desire to know the steps of collecting primary data and secondary and most importantly how to use Q-Slope Classification System to delineate and analyse accurately the stability and instability...

30 September 2023 5,159 2 View

What is a good questionnaire/survey to find out awareness on physiotherapy treatment among adults?

Questionnaires or surveys done regarding any illness or condition would be helpful. Specifically treatment awareness for cerebral palsy or such disability.

20 April 2022 6,204 8 View

Hello, can any one help to identify the following plant species?

Pictures were taken in Mbeya, Tanzania

21 July 2021 8,114 6 View

Anyone with Daily Index Data for Kenya's Securities Exchange Market?

I am working on a study to test the efficiency of Kenya's securities exchange market. Should anyone have the NSE daily index data (as far back as you can and as recent as it can get - the...

07 March 2021 8,444 3 View

Is there any documentation study on the correlation between age and vulnerability to the coronavirus disease?

Evaluation of vulnerability of school children below 10 years being investigated.

06 September 2020 6,227 3 View

Are there any research and statistical data on the causes of coronavirus disease?

Causes of coronavirus disease under investigation.

06 September 2020 3,912 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View