I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?
Dear friend Sivasakthi Paramasivam
The s-CM5 sensor chip is commonly used for surface plasmon resonance (SPR) analysis to immobilize proteins for binding studies. The chip surface is coated with a thin layer of carboxymethylated dextran, and proteins are immobilized on the surface through covalent coupling or non-specific adsorption.
To store the protein immobilized s-CM5 sensor chip, it is recommended to use a buffer that is compatible with the immobilized protein and will not cause significant degradation or denaturation of the protein. HBS-EP buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, and 0.005% Tween-20) is a commonly used buffer for storing immobilized proteins on SPR sensor chips. This buffer helps to maintain the stability and activity of the immobilized protein over time.
For long-term storage of the protein immobilized s-CM5 sensor chip, it is recommended to store the chip in a sealed container or bag with the HBS-EP buffer at 4°C or -20°C. The storage time depends on the stability of the immobilized protein, but it is generally recommended to use the chip within a few weeks to a few months of immobilization to ensure optimal binding activity.
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