What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

What are your dreams for ICM?

Congratulations & Good Wishes ICM Celebrates 3rd Anniversary If there is ever a corporate history written on ICM, it will start with you and end with you. You are not just another amazing...

24 July 2020 6,454 3 View

Where to purchase Ba(MnO4)2 for Hollandite synthesis?

Dear all, for synthesis of Ba-Hollandite (BaMn8O16) described in: Cheng, S., Lin, J. T., Ocelli, M. L., & Robson, H. E. (1992). Expanded Clays and Microporous Solids (p.335 & 337), I need...

30 June 2020 3,846 7 View

Is there a German version of the WHO Workplace Violence in the Health Sector Questionnaire?

We are currently working on a project on health promotion of employees in the home care sector. To asess the current burden of this specific group, we would like to measure the degree of workplace...

29 June 2020 7,752 2 View

Can anyone please explain about the significance of powder XRD in characterizing phosphor materials?

Greetings everyone, hope you are all doing well. I just want a clear definition about how we can analyze the structural morphology of RE doped phosphors with powder XRD and what are all the...

12 May 2020 6,567 4 View

What is the best way to do immunohistochemistry on perfused gut tissue from rat?

We have recently collected perfused whole mount sections of the rat gut, containing the duodenum, ilium and colon. Following perfusion the sections were cut longitudinally but kept all in one...

02 April 2020 328 3 View

Is it possible that in the future the softwares of any spectroscopy system can run on a dedicated OS on dedicated computers?

I'm asking this question because current modern Spectroscopy machines, which are fully based on digital electronics can become obsolete or their upgrading is extremely expensive everytime there...

11 November 2019 6,531 3 View

I need expert feedback. Can someone test my questionnaire?

Hello everybody, I need feedback on my survey before I publish it. I would be happy if someone could test it first. The survey is (unfortunately) in German. It is about competencies of founders in...

26 July 2019 5,288 3 View

What is the duration of pollinator activity after they emerge?

I have been looking for information about the duration of the activity of pollinators after they emerge. I am aware that this will vary among species. However, there may be some average value. I...

06 May 2019 6,458 4 View

How to synthesize pure feitknechtite?

04 May 2019 5,432 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View