Hi there, is there anyone who knows about CYP21A2 gene specific primers for routine PCR?

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No title

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Hasnaa Aboelwafa

The ideal sequences for for the primer, between 18-24 bp , In gene bank web site you can select the optimum length, and position for each primers.

Alireza Tafazoli

Dear Hasnaa

Thanks for your information. I will check it.

Paul Rutland

try submitting your sequence into exonprimer and it should come up with primer sets for all exons which can be further checked using primerblast if you think that is necessary

https://ihg.helmholtz-muenchen.de/ihg/ExonPrimer.html

Thank you Paul. i will do it and see the results.

Tasnuva Kabir

Keep in mind that your primer is specific to your mRNA region when you order SYBR green primers (but TaqMan should be ok). You can run a primer blast NCBI. I noticed anything non-specific upto 400 bp will give you false positive results. You can test specificity by melt curve and running your PCR product in DNA gel.

Thank you Tasnuva.