Tasnuva Kabir

3 Questions 6 Answers 0 Followers

Questions related from Tasnuva Kabir

What is the best way to ensure that all data points start at the same point at time point 0 in incucyte proliferation assay by live cell imaging?

Live cell imaging using IncucyteZoom is the best way to measure effect of drugs on rate of cell proliferation. I noticed that despite of how accurate we try to count the cells using automated...

13 September 2021 7,189 3 View

Can anyone please tell me how many times can I passage LX2 cells without changing their phenotype?

I am using LX2 cells to understand the role of HSCs in Hepatocellular carcinoma.

04 May 2017 762 2 View

If a miRNA structure is modified by addition of a methyl Fluoro group or LNA, does this affect the PCR efficiency of the TaqMan real time probes?

We have modified the miRNA structure in different ways as mentioned in the question at multiple nucleotides of the mature MiRNA strand duplex mimics. Is there a chance the SYBR primers may be a...

11 November 2016 1,945 1 View