The KO mES cells are growing very slow. I passaged 10 days back. The confluency is about 35%.So should I wait until it reaches 85% confluency or should I put more feeders over them? I am afraid they would differentiate. Please let me know what should be my strategy to passage them? Thanks for your time.
Harsh Panwar
You may need to check 1. Passage number of your cells; better take passage no. 2-10. 2. Check your media composition and culturing conditions. 3. Initial support with 15%FBS, instead of 10% may help to resolve your problem. Best Wishes
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