Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Do I have the right PCR cycler settings?

Hello. Someone designed primers and cycler settings for me and it doesn't work. I do hot start PCR with touchdown, I detect Babesia sp. DNA in Acari. The primers are F: GYYTTGTAATTGGAATGATGG, R:...

01 March 2020 3,949 3 View

How do I determine differences between two hemp seed samples to determine if they are genetically different strains or the same strain?

Are there currentlly any methods developed for determining the differences in genome between two samples?

08 August 2019 4,594 2 View

My cells started to form clumps during passaging. I tried almost everything to reduce them. Do you have any suggestions how to solve this problem?

My cell cultures (HeLa S3, mouse kidney cells, WB-neo, WB-ras) strarted to form clumps during passaging so it is not possible to count them properly. I TRIED 1. make PBS without Mg and Ca...

14 January 2019 9,398 6 View

How to remove the Percoll from the sample?

I use density gradient centrifugation with Percoll to extract bacteria from the bentonite sample. It is working quite well, but I need to remove the Percoll from the bacterial extract at the end...

25 July 2018 981 3 View

How influence the concentration of oxygen growth of oxide layer and properties of oxide layer itself due to thermal oxidation?

Hello, I'm working on my diploma work on thermal oxidation of TiNb alloy.In my work I have oxidized titanium by two procedures thermal oxidation in laboratory atmosphere and in atmosphere of...

22 May 2018 1,704 5 View

F.I.S.H probe: Can be used as a probe a pcr labeled product with 600-1000 bps size?

Is it nessessary to be inserted in a BAC clone?

05 June 2016 3,512 1 View

I'm looking for taxonomic keys (for genera) - for identification of grasshoppers from Borneo (Malaysian part). Please, do you know some keys?

01 March 2016 6,885 5 View

Does anyone know whether a chromatography mode exists when both the stationary and mobile phase (developing solvent in TLC) are nonpolar?

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10 October 2012 8,485 11 View

Stimulation of CD8+ CTL with specific antigen in vitro

How to increase effectivity of CTL stimulation with synthetic peptide epitopes.

13 October 2011 2,226 1 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View